Mammalian Cell Engineering for Applications In Vitro

Mammalian Cell Engineering for Applications In Vitro

  • Synthetic Biological Circuits.
    Synthetic Biological Circuits.

The project is dedicated to rational design and engineering of mammalian cells augmented with complex functionalities, for applications in non-medical biotechnology. 

The two application areas include rational forward design of bioproducing mammalian cell lines, and the development of sentinel (reporter) cells for highly-informative drug discovery assays. In the former, we combine the laters tools of genetic engineering such as synthetic biology and automation, with the recent genome editing approaches and rational in situ control of the bioproduction process on a single cell level, to achieve end-to-end workflow for biomanufacturing cells without the need for screening. In the latter project, we harness the power of biomolecular computing to engineerer reporter cells that detect multiple drug effects in parallel. This dramatically increases the information content of a cell-based drug screening assay without reducing the throughput. 

Read the interview with the Benenson-Group in the newsletter MOLEKULAR-ia (issue February 2016, Vol. 2). 


M. Dastor, J. Schreiber, L. Prochazka, B. Angelici, J. Kleinert, I. Klebba, J. Doshi, L. Shen, Y. BenensonA Workflow for In Vivo Evaluation of Candidate Inputs and Outputs for Cell Classifier Gene Circuits“ ACS Synth. Biol. (2017). [Link]
L. Prochazka, Y. Benenson, P. W. Zandstra “Synthetic gene circuits and cellular decision-making in human pluripotent stem cells“ Curr. Opin. Syst. Biol. (2017). [Link]
J. Schreiber, M. Arter, N. Lapique, B. Haefliger, Y. BenensonModel‐guided combinatorial optimization of complex synthetic gene networks“ Mol. Syst. Biol. 12, 899 (2016). [Link]
Y. BenensonComplexity from Simple Building Blocks: Engineering Large-scale Information-processing Networks from Molecules“ Chimia 6, 392 (2016). [Link]
B. Häfliger, L. Prochazka, B. Angelici, Y. BenensonPrecision multidimensional assay for high-throughput microRNA drug discovery“ Nat. Commun. 7, DOI: 10.1038/ncomms10709 (2016). [Link] [More Information]

Who works with whom?

Prof. Yaakov Benenson from the ETH Zürich (Dept. Biosystems Science and Engineering) leads this project and works with Raffaele Altamura (PhD-student). 


Read more about the Benenson-Group here.


The Benenson-Group works in collaboration with projects led by Sai Reddy, Jörg StellingSven Panke, Martin Fussenegger, Gebhard Schertler, Botond Roska and Cornelia Palivan.